RjHan:SD rat CST3


Available to order

International strain nameRjHan:SD rat CST3
Alternative namerCST3
Strain typeEndonuclease-mediated
Allele/Transgene symbolZygote electroporation of gene-specific gRNA, Cas9 and ssODN template was used to introduce M88Q point mutation.
Gene/Transgene symbolCst3

Information from provider

ProviderJana Kopkanova
Provider affiliationTransgenic and Archiving Unit, Institute of Molecular Genetics of the Czech Academy of Sciences
Genetic informationcystatin C (Cst3, CYSC, MGC105556) gene; mutant rats were generated by zygote electroporation of gene-specific gRNA (5´-CTATTATTTGGATGTGGAGA-3´), Cas9 and ssODN (5´-CAGTTAGTCAAATTTGTCTGGGACTTGGTACATGTAGTTCGGCCCtgCTCCA CgTCCAAATAATAGTTTATTCCAGCCACAAGCTACAGTGAAAAAGAG-3´) template was used to introduce M88Q (p.Met88Gln) point mutation into the 2nd coding exon. Positive founders were identified by RFLP analysis.
Phenotypic informationHomozygous:
not analyzed

not analyzed
Breeding history2 generations crossed to RjHan:SD background
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic
Animals used for archivingwild-type rat Sprague-Dawley , heterozygous rat Sprague-Dawley
Stage of embryos2-cell

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Practical information

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