Sprague Dawley rat Clcn4em2(A549V)Ics


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International strain nameSprague Dawley rat Clcn4em2(A549V)Ics
Alternative nameSprague Dawley rat Clcn4em2(A549V)Ics
Strain typeEndonuclease-mediated
Allele/Transgene symbolClcn4em2(A549V)Ics
Gene/Transgene symbolClcn4

Information from provider

Provider Cure CLCN4
Provider affiliationCure CLCN4
Genetic informationThe A549V mutation (GCT>GTT; corresponding to the human A555V mutation) was introduced in rat Clcn4 gene using a CRISPR/Cas9 approach. The ribonucleoprotein complex composed by a guide TGGTAACAGCAGCTGCCATC and the Cas9 protein was co-electroporated with a single stranded donor DNA (gtgtctctggtagtcatcatgtttgaactgactggaggtctggaatatattgtaccgctcatggcagttgctgttaccagcaagtgggtggctgatgcctttgggaaagaagggatttatgaagc) baring the A549V mutation and 2 additional silent mutations, one of them mutating the PAM sequence and both leading to a new BsrBI diagnostic restriction site. Pups were genotyped with the following primers: gggataaaattatgatctgtaagct and gagtctccacatcctccacagtcat. In the presence of the mutation, this 390 bps PCR fragment will be digested in 2 fragments of 170 and 220 bps by BsrBI
Phenotypic informationHomozygous:
Not done

Smaller animals, especially hemizygous males. Dental malocclusion and locomotor problem found in a male
Breeding historyThe line was kept on a Sprague Dawley genetic background
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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